John Stamos - Public Service Announcement
Assay Endpoint
Advantages of Ex Vivo Analysis of Programmed Cell Death (EVA-PCD?) over older proliferation-based methods
- Programmed cell death end points are more reflective of chemotherapy effects in vivo
- Peer-reviewed published correlations with response and survival.
- Drug and disease specific (robust)
- Rapid, versatile, and applicable to biologic agents as well as radiation
- Cytolysis measured in dividing and non-dividing populations
- Accurately distinguishes tumor from non-tumor elements
- Serial dilutions provide cytotoxic drug dose-response curves
Additional Cell Death Assays-ATP & MTT
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Rational Therapeutics™ employs multiple methodologies based on cell death endpoints, including the delayed loss of membrane integrity , ATP* content & MTT**signal as components of the EVA-PCD™ service
*The ATP assay is a luminescent short term suspension culture assay which functions by measuring the metabolic activity of living cells.
** The MTT assay is a colorimetric short-term suspension culture assay which functions by measuring the metabolic activity of living cells.
The different assays are applied according to specimen quality.
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